To understand the behavior of cells towards their environment, their interaction with other cells, bacteria or viruses and their surrounding during their development forms part of current research. Understanding ongoing processes such as differentiation, signaling and interaction can help in further investigating altered cell functions such as tumor formation or viral attacks. Processes that occur inside cells or in the membrane are still largely unknown and under extensive observation. Scanning electron microscopes are ideal instruments to observe cellular or tissue structures at high resolution.
In addition, CLEM (correlative light and electron microscopy) helps researchers to understand the processes mentioned above as it combines the information from a fluorescent wide-field image with a high resolution SEM image. Besides the possibility to choose the region of interest for a more detailed study, also an overview of a tissue can be taken into consideration to perform several detailed studies next to each other or in a chosen region of interest.
Delphi is the world's first fully integrated CLEM solution that enables fast correlative microscopy with unique overlay precision. Correlative light and electron microscopy (CLEM) is the combination of fluorescence and electron microscopy and thus a powerful technique to combine the power and versatility of fluorescence microscopy to acquire fluorescence images and to easily place these images with functional information in a structural context.